Coding

Part:BBa_K5398601:Design

Designed by: qijia ren   Group: iGEM24_NAU-CHINA   (2024-09-10)


Mfp6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none.


Source

The nucleotide sequence is determined by DNA sequencing analysis and find to be identical to that in the GenBank database (DQ351537.1). Besides, the Mfp6(363 bp) was cloned from the pETDuet-1-Mfp6 vector which was synthesized by the company.

References

[1] Nicklisch SC, Das S, Martinez Rodriguez NR, et al. Antioxidant efficacy and adhesion rescue by a recombinant mussel foot protein-6[J]. Biotechnol Prog., 2013, 29(6):1587-1593.

[2] TAN D, ZHAO J P, RAN G Q, et al. Highly efficient biocatalytic synthesis of L-DOPA using in situ immobilized Verrucomicrobium spinosum tyrosinase on polyhydroxyalkanoate nano-granules [J]. Appl. Microbiol. Biotechnol., 2019, 103(14): 5663-78.

[3] YAO L, WANG X, XUE R, et al. Comparative analysis of mussel foot protein 3B co-expressed with tyrosinases provides a potential adhesive biomaterial [J]. Int. J. Biol. Macromol., 2022, 195: 229-36.